Famous Autographs

Authentic Autographed

All About Celebrity Autographs

The celebrity autograph industry is big business nowadays. If you are thinking of buying a celebrity autograph, the most important thing to remember is to buy from a reputable source to avoid disappointment. This will increase the odds that you are buying an Authentic Autograph. Some other expert buyers and sellers would advise to avoid buying from sellers who sell at a fixed price and are members of exclusive or Rare Autograph societies. These dealers tend not to have the seller's best interests at heart and sometimes the prices are ridiculously high and you end up getting ripped off. This means that if you are planning on selling on your items you will be very unlikely to make a profit.

 

The best place to buy celebrity autographs is to go to an autograph auction site where prices generally sell for a bit less than retail prices. This is ideal if you are looking to make a profit from investing. eBay has made the celebrity autograph business expand dramatically as it has made it much more accessible and easy to buy on the internet all over the world.

 

If you are looking to sell your autographs to a dealer then you need to take into account that they have overhead expenses as a result of running a business on a day-to-day level. This generally means that the dealer will only pay half of the retail price in order for them to be getting a good deal. Prices do vary, some pay slightly more than the retail price and some slightly less.

 

You need to be aware that if you attempt to sell to dealer-members who belong to high-brow or rare autograph societies then they tend to pay even less in order to cover costs lost due to the lack of proper sales and marketing promotion. So be aware who you sell your items to.

 

If you are in the situation where you need immediate money and not at the highest price, then a quick sale to a dealer might be best. If this isn't for you then you can try selling your autographs at an autograph auction. Instead of only realising 50% of the value of your items like when you sell to an autograph dealer, an auction allows you to get anywhere from 60%-80% of the current price.

 

The most important to keep in mind when buying or selling celebrity autographs is whether or not they are authentic. Autograph authentication is a hotly disputed genre of the memorabilia industry. There are lots of authentication companies out there that work to bring down the risk of a buyer being ripped off by a fake autograph and the margin of error is significantly low. However, other experts claim that you should not deal with the authentication companies at all and recommend that you instead get a knowledgeable dealer or collector to assess your item.

 

Lots of people buy celebrity autographs or signed memorabilia in order to sell it on as an investment. The best way to make the best investment is to buy one-of-a-kind or two, three, four or five of-a-kind collectible items. It is all a case of supply or demand; it depends what celebrity is popular at the time etc.

 

If you are looking to start a celebrity autograph collection or add to an already established one, then why not check out Genuine Memorabilia.

 

 

 

 

About the Author

What is an authentic autographed Andre Johnson red jersey worth?

It could be worth anything, it depends on where you got the autograph and things like that. But, i would say it is probably more than 200 if im thinking correctly. i personally have a ton of sports memoribilia and i have come to know it all dpends

TNA Kurt Angle Authentic Autographed Card ONLY 300 made Very Rare

Authentic Autograph

Ne Autograph

Ne Autographs Contact - Ne Autographs Contact

Your Poster comes with a Lifetime Money Back Guaranteed Certificate of Authenticity.
Plain and simple we thoroughly inspect any item we sell, to guarantee it validity. If we did not get it our self we go through painstaking inspection to make sure your poster is authentic. Customer satisfaction is our first priority!

What you see on the website is only a snapshot of what we sell. Due to the unique nature of our business, sometimes it can take us months to authenticate and negotiate for a piece. By the time we receive the piece, there can be several parties interested in purchasing it. As a result, a high percentage of our pieces sell in the first 48 hours. The best way to obtain quality pieces is to contact us with a ?wish list?.

Ne Autographs Posters Many of our posters contain iconic images and are HIGHLY sought after by collectors. Some notable genres and titles include: James Bond, Cartoons, “Bad Girl”, Westerns, Horror, Science Fiction, Hitchcock, Jaws, The Blues Brothers, Star Wars, Oceans Eleven, The Godfather, Blade Runner, Scarface, A Clockwork Orange, Pretty Woman, Breakfast at Tiffany's, etc.

The Autograph Source is located in downtown Aspen, Colorado. Our location gives us exposure to many of the modern celebrities and their agents. The simple fact that many of the autographs that we sell are from people who pass through our retail location gives our customers absolute confidence that our pieces are real.

Ne Autographs Movie

Over the years original vintage movie posters have been produced in various shapes and sizes. Before the 1980’s and the demise of the small town movie theatres (as a result of the monopolization of the industry by companies like Showcase cinemas) also meant the extinction of all the breath-taking movie posters such as the lobby cards, window cards, insert, half sheets and the traffic-stopping 3, 6 and 24 sheets. The primary Movie Poster that survives today is the standard one sheet. Below is a glossary of the most popular vintage movie posters that once played a major role in the promotion and exhilarating anticipation of upcoming movie events.

Where were these autographs obtained?
These autographs were obtained in Los Angeles and NYC press events, and special signings for promoting the films. Or at premieres, promos and private collections, obtained by myself and very reputable dealers we purchase from. These are genuine Hand Signed autographs. Not reprints, preprints, copies or computer facsimiles. Please do not be fooled by any of the above by countless sellers online. We only offer authentic material.

Don't miss out, learn more:
Ne Autographs
About Ne Autograph
About Ne Autographs
Ne Autographs
Ne Autograph Posters

About the Author

Ne, Autographs, Ne AUtograph posters

How much do you think I would get for this Ray Allen autograph, and what should the starting price be?

Hi!
I just won an Authentic Autographed Ray Allen picture in a raffle, and I'd like to try selling it on eBay. It's an 8 x 10. How much do you think I could get for it? What should the starting price be? Thanks!

I wouldn't exactly bid on a price, unless I'm a real expert on appraising autographed memorabilia photos. My suggestion is search over the internet or directory for an expert who appraises autographs and memorabilia. They generally have an idea how much it's worth. You wouldn't want it to overprice or under-price the autograph now would you?

Search over the net for similar Ray Allen autographs and compare how much it's worth, that will give you a rough idea also. Lots of luck!

Avenged Sevenfold Original Authentic Autograph for sale!

Autograph Authentic

Autographed Sports Memorabilia. How to determine their Value

Sports Autographs are one of those unique hobbies that many people start at a very young age. Whether it be Michael Jordan flying in for dunk or Joe Montana finding a receiver for a game-winning touchdown, these are the memories that remain with us for years and instantly turns us into fans of the player and the game.

 

As we continue to watch with amazement the highlights and the special gifts of athletes to thrill so many spectators, we cannot help but want to savor the moment and accomplishments of that athlete. Autograph collecting allows us to remember fondly the countless special plays athletes make during their illustrious career. So how do we determine the value of sports autographs?

 

The value of a sports autograph vastly depends on numerous factors. First and foremost, supply and demand. A Michael Jordan sports autograph is far more sought after than a John Paxson autograph. The memorabilia the athlete has signed also plays a valuation role. A signed game jersey, basketball etc. has far more value than a signature on a piece of paper or coffee cup. The condition of the signed item is also important. An item that has no alterations enhances the value of the sports autographs. Next, how was the Autograph Signed? A pen/ marker signed name tends to write clearly and smoothly and usually does not smear or fade in the future. Pencil signatures tend to fade and do not create a clear signature as a pen. Another important value point is where did the sports autograph come from. A signature from a vendor who exclusively contracts with the athlete can provide solid documentation of authenticity and usually verification. This will likely drive the price of the autograph, but you will be assured of an investment that will rise in the future.

 

Sports autographs are an exciting hobby. If done right and with a little research, you can find top-notch athletes on hundreds of great memorabilia items that will be sure to capture your competitive spirit as well provide a great investment vehicle.

About the Author

Ameesh Morjaria, owner of Autographed Sports Memorabilia company All Authentic Autographs

www.AllAuthenticAutographs.com

How to tell if an autograph is authentic if it's through the mail?

I was going to send one of my favorite TV hosts a fan letter when I saw you could ask for their autograph as well (on the website that provided the address). I'm just worried that if I receive the autograph it will be fake. Is there any way to tell if an autograph is real or fake if you received it in the mail? Thanks!
Every time I look on google I type in their name then "autograph," but I come up with a ton of sports names. It doesn't even matter if I put the person's name in.

Does anyone have a picture of an autograph of Joel McHale, the host of The Soup?
Thanks.

There's two ways to check if it's real.

1. Turn the paper slightly to see if it's written with a real pen.
2. Compare it to other autographs you see online.

WWE Autograph Collection (Current) - (Authentic)

Psa Dna Authentic

STANDARDISATION AND EVALUATION OF HERBAL DRUG FORMULATIONS

STANDARDISATION AND EVALUATION OF HERBAL DRUG FORMULATIONS

1Sunita Panchawat, 1Kamal Singh Rathore, 2Dr.S.S.Sisodia, 3Dr. R.K.Nema

1BN PG Girls College of Pharmacy, Udaipur-Raj.INDIA 313002

2BN PG College of Pharmacy, Udaipur

3Rishiraj College of Pharmacy, Udaipur

Email:kamalsrathore@yahoo.com

Mobile: +919887111211(Sunita P.); +919828325713(Kamal)

 

Herbal Drug Standardization and Evaluation:- In recent years, there has been great demand for plant derived products in developed countries. These products are increasingly being sought out as medicinal products, nutraceuticals and cosmetics. (1) There are around 6000 herbal manufacturers in India. More than 4000 units are producing Ayurveda medicines. Due to lack of infrastructures, skilled manpower reliable methods and stringent regulatory laws most of these manufacturers produce their product on very tentative basis. (2)

   In order to have a good coordination between the quality of raw materials, in process materials and the final products, it has become essential to develop reliable, specific and sensitive quality control methods using a combination of classical and modern instrumental method of analysis. Standardization is an essential measurement for ensuring the quality control of the herbal drugs. (3) “Standardization” expression is used to describe all measures, which are taken during the manufacturing process and quality control leading to a reproducible quality. It also encompasses the entire field of study from birth of a plant to its clinical application. It also means adjusting the herbal drug preparation to a defined content of a constituent or a group of substances with known therapeutic activity respectively by adding excipients or by mixing herbal drugs or herbal drug preparations.(4) “Evaluation” of a drug means confirmation of its identity and determination of its quality and purity and detection of its nature of adulteration.(5)

    Standardization of herbal drugs is not an easy task as numerous factors influence the bio efficacy and reproducible therapeutic effect. In order to obtain quality oriented herbal products, care should be taken right from the proper identification of plants, season and area of collection and their extraction and purification process and rationalizing the combination in case of polyherbal drugs.(3)

    The Standardization of crude drug materials includes the following steps:-

1. Authentication: - Each and every step has to be authenticated.

a)      Stage of collection.

b)      Parts of the plant collected.

c)      Regional status.

d)     Botanical identity like phytomorphology, microscopical and histological analysis (characteristic of cell walls, cell contents, starch grains, calcium oxalate crystals, trichomes, fibers, vessels etc).(6) Various histological parameter studies are:-

1. Leaf constant: - Palisade ratio, Vein islet number, Vein termination, Stomatal number, and Stomatal index.
2. Trichomes.
3. Stomata.
4. Quantitative microscopy.
5. Taxonomical identity.
6. Foreign matter.
7. Organoleptic evaluation.
8. Ash values and extractive values.
9. Moisture content determination.
10. Chrometographic and spectroscopic evaluation.
11. Heavy metal determination.
12. Pesticide residue.
13. Microbial contamination.
14. Radioactive contamination.

The herbal formulation in general can be standardize schematically as to formulate the medicament using raw materials collected from different localities and a comparative chemical efficacy of different batches of formulation are to be observed. The preparations with better clinical efficacy are to be selected. After all the routine physical, chemical and pharmacological parameters are to be checked for all the batches to select the final finished product and to validate the whole manufacturing process. (6)

The stability parameters for the herbal formulations which include physical, chemical and microbiological parameters are as follow:

Physical parameters include color, odor, appearance, clarity, viscosity, moisture content, pH, disintegration time, friability, hardness, flow ability, flocculation, sedimentation, settling rate and ash values.

Chemical parameters include limit tests, chemical tests, chemical assays etc.

Chromatographic analysis of herbals can be done using TLC, HPLC, HPTLC, GC, UV, GC-MS, fluorimetry etc.

Microbiological parameters include total viable content, total mold count, total enterobacterial and their count. Limiters can be utilized as a quantitative or semi quantitative tool to ascertain and control the amount of impurities like the reagents used during abstraction of various herbs, impurities coming directly from the manufacturing vessels and from the solvents etc.

 

GUIDELINES FOR HERBAL DRUG STANDARDISATION

WHO Guidelines:-

The subject of herbal drug standardization is massively wide and deep. The guidelines set by WHO can be summarized as follows:-

• Reference to the identity of the drug. Botanical evaluation- sensory characters, foreign organic matter, microscopical, histological, histochemical evaluation, quantitative measurements etc.
• Reference to the physicochemical character of the drug. Physical and chemical identity, chromatographic fingerprints, ash values, extractive values, moisture content, volatile oil and alkaloidal assays, quantitative estimation protocols etc.
• Reference to the pharmacological parameters, biological activity profiles, bitterness values, hemolytic index, astringency, swelling factor, foaming index etc.
• Toxicity details- pesticide residues, heavy metals, microbial contamination like total viable count, pathogens like E.coli, Salmonalla, P.aeroginosa, S. aureus, Enterobacteria etc.
• Microbial contamination.
• Radioactive contamination.

  

Modern herbal ayurvedic monographs

In the modern herbal ayurvedic monographs the standardization parameters are discussed in a comprehensive way. According to the modern ayurvedic monograph the quality control protocols include the following:

The synonyms, publication related to the plant, constituents present, analytical methods.

Descriptive evaluation: Description of the drug, phytomorphological, microscopical, organoleptic evaluation, foreign matter etc.

 

WHO GUIDELINES MONOGRAPH TITLE (7)

 Botanical: - Sensory evaluation, Foreign matter, Microscopy measurement.

Physicochemical TLC: - Ash, Extractable matter, Water content and volatile matter, Volatile oils.

Pharmacological: - Bitterness value, Haemolytic activity, Astringency, Sterling index, Foaming index.

Toxicological: - Pesticide residue, Arsenic, Metals.

Microbial contamination: - Total viable count, Pathogens, Aflatoxins, Radioactive contamination.

 

STANDARDIZATION OF HERBAL DRUG/PRODUCTS

 Commercial production of herbal medicines and their trade are fast growing sector of industry today, due t6o increasing demand of medicinal plants; the supply line is adversely affected leading to the adulteration and substitution for genuine drugs.

1.      Fluorescence quenching:- When a plant extract is spotted on a fluorescent silica gel layer and exposed to UV light, it appears as spot on a fluorescent background, thus causing quenching and is directly proportional to concentration of the extract. Silica gel GF plate was used as an adsorbent for fluorescence quenching. Solvents taken- hexane toluene, ether, ethyl acetate, butanol, methanol and water.(8)

2.      Use of fingerprinting and marker compounds for identification and standardization of botanical drugs:- Chemical and chromatographic techniques may be used to aid in identification of an herbal material or extract. Chromatographic technique such as HPLC, TLC, GC and capillary electrophoresis and spectroscopic methods such as IR, NMR, and UV-may also be used for fingerprinting. DNA fingerprinting has been widely used in many species, e.g. DNA fingerprinting of Panax species and their adulterants.(9) Marker compounds may be used to help identify herbal materials, set specifications for raw materials, standardize botanical preparations during all aspects of manufacturing processes and obtain stability profiles.(10)

3.      Densitometric thin layer chromatographic determination of aescin in an herbal medicinal product containing Asculum and Vitis dry extract:- A TLC method is developed to analyze the total saponin content, also referred to as the aescin content, in an herbal medicinal product containing two dry extract in capsules. After a purification step using C(18) solid phase extraction, the samples are analyzed on a silica gel HPTLC plate with the upper layer of a mixture of acetic acid/water/butanol(10/40/50v/v/v) as the mobile phase. Spots are visualized by spraying with anisaldehyde reagent and heating the plate for 5-10 min.(100-105oc) and measured at a wavelength of 535 nm.(11)

4.      Determination of stigmasterol, beta-sitosterol and stigmastanol in oral dosage forms using HPLC with evaporative light scattering detection: - A validated and repeatable HPLC method with online evaporative light scattering was developed for the analysis of two sterols, stegmasterol, beta-sitosterol and a stanol found to be common in many herbal formulations and health care supplements. This method was used to assay commercially available products formulated as oral dosage forms purported to contain African potato and associated sterols and stanol. (12)

5.      Elemental analysis of herbal preparations for traditional medicines by neutron activation analysis with the kO standardization method: - Medicinal herb preparations prescribed for specific treatment purposes were purchased from markets and were analysed by instrumental neutron activation analysis with kO standardization. 500-700 mg of each sample was palletized under a pressure of six tones and irradiated together with monitors for alpha and neutron flux ratio determination for about 6h in a thermal flux of 2.29 x 10(12) n/cm2/s.(13)

6.      Liquid chromatography UV-determination and liquid chromatography-atmospheric pressure chemical ionization mass spectrometric characterization of sitosterol and stigmasterol in soyabean oil:- A narrow bore HPLC-UV method was developed for the analysis of two of the more abundant naturally occurring phytosterols in vegetable oils: sitosterol and stigmasterol. The method enabled detection of the compounds at a concentration of 0.42 µ/ml and quantization at concentration of 0.52 and 0.54 µ/ml for sitosterol and stigmasterol, respectively.(14)

7.      Simultaneous determination of cinnamaldehyde, eugenol and paeonol in traditional Chinese medicinal preparations by capillary GC-FID: - A capillary GC method was established for simultaneous determination of cinnamaldehyde(CNMD), eugenol(EL) and paeonol(PL) in two traditional Chinese herbal medicinal preparations, Weitongding tablet (WTDT) and Guifu Dihuang pill (GDHP). The assays were based on a programmed temperature GC in a 30 m x 0.53 mm capillary column with nitrogen as carrier and FID detector. Good linearity were obtained over ranges of 0.45-0.452 mg/l CNMD, 0.31-0.625 mg/l EL, and 0.30-610 mg/l PL, respectively. (15)

8.      HPTLC fingerprinting of marketed formulation containing Shankhpushpi: - These are the important Ayurveda formulations used for perinatal care of mother and child health. Standardization of churnas was carried out by organoleptic study, phytochemical analysis; qualitative organic and inorganic analysis, thin layer chromatography, UV- visible spectrophotometer and HPLC fingerprint studies. Qualitative organic analysis of both the churnas revealed the presence of alkaloids, steroids, phenols, tannins, glycosides, resins, saponins and flavonoids.(16)

 

                       EVALUATION OF HERBAL DRUG/PRODUCTS

1.      Biological parameter (bioassay):- It is well established that the biological potency of the herbal constituents is due to not one but a mixture of bioactive plant constituents and the relative properties of a single bioactive compound can vary from batch to batch while the biological activity remains within the desirable limits. (1) Some of the examples are:_

a.         Evaluation of adaptogenic activity profile of herbal preparation: - Adaptogens help the body to come up with stress and enhance general health and performance.  AVM is an herbal formulation. Composition- Emblica officinalis, Withania somnifera, Asparagus racemosus, Ocimum sanctum, Tribulus terrestris and Piper longum. AVM shows significant antistress, immunomodulatory and anabolic activities in different animal models there by proving a promising adaptogen. (17)

b.        Evaluation of antioxidant activity of herbal products: - A new test method for measuring the antioxidant power of herbal products, based on solid phase spectrophptometry using tetrabenzo-b, f, j, n, l, 5, 9, 13- tetraazacy- clohexadecin- Cu (II) complex immobilized on silica gel is proposed. The method represents an alternative to the mostly used scavenging capacity assays. The method was approved in the analysis of the most popular herbal beverages and drugs Echinacea determined spectrophotometrically.(18)

c.         Evaluation of microbial contamination reduction on plants through technological process of decoction and spray dry: - The technological process of raw material has many stages, generally, adverse to microbial growth, but its complete elimination depends on the initial and work condition utilized. The aim of this work was to verify the microbial contamination, such as extractive solution (SE) and spray dried extract (PSA) with the purpose of evaluating the decrease of contamination after the decoction and the spray dry. The microbiological analysis of the products was performed by total plate count and MPN coliform. (19)

d.        Evaluation of nitric oxide scavenging activity of selected medicinal plants used in inflammatory diseases: - Four traditional medicinal plants, namely Ventilago madraspatana Gaertn., Rubia cordifolia Linn., Lanatana camara Linn. And Morinda citrifolia Linn. Were selected for a study on the inhibition of nitric oxide (NO), a key mediator in the phenomenon of inflammation, signifying the presence of effective anti-inflammatory constituents therein. Plant samples were extracted with different solvents for evaluation of their inhibitory activity on NO produced in vitro from sodium nitroprusside, and in LPS- activated murine peritoneal macrophages, ex-vivo.(20)

e.         The lipid peroxidation inhibitory activity:- The reaction mixture contained mice liver homogenate (0.2 ml, 10% w/v) in 0.15 KCl, KCl (0.1 ml, 150 µm), Tris buffer (0.4 ml, Ph 7.5) and various concentration of test extracts. In vitro lipid peroxidation was initiated by addition of Feso4.7H2O (0.1 ml, 10 µm). The reaction mixture was incubated at 37o for 1 h. After the incubation period, reaction was terminated by addition of thiobarbituric acid (TBA-2 ml, 0.8%) and by heating the contents for 15 min. for development of colored complex. The tubes were then centrifuged at 4000 rpm for 10 min. and cooled. The % inhibition of lipid peroxidation was determined by comparing the results of test compound with those of control not treated with extracts by monitoring the color intensity at 532 nm. Gallic acid was used as a positive control. (21)

2.      Evaluation of marketed polyherbal antidiabetic formulatios using biomarker charantin: - Charantin is one of the phytoconstituents present in Momordica charantia. It is well known to possess antihyperglycaemia, anticholesterol, immunosuppressive, antiulcerogenic, antispermatogenic and androgenic activities. HPTLC method is fast, precise, sensitive and reproducible with good recoveries for standardization of polyherbal formulations. The recovery values of charantin were found to be about 98.89%. (2)

3.      In vivo and in vitro evaluation of hair growth potential of Shoe flower: - The leaves and flowers of Hibiscus rosa-sinensis are used as promoters of hair growth and as an aid in healing of ulcers. Petroleum ether extract of leaves and flowers of the plant was evaluated for the potential growth in vivo and in vitro methods. In vivo, 1% extract of leaves and flowers in liquid was applied topically over the shaved skin of albino rats and monitored and assessed for 30 days. The length of hair and different cyclic phases of hair follicles, like anagen and telogen phases were determined at different time periods. In vitro, the hair follicles from albino rat neonates were isolated and cultured in DMEM supplemented with 0.01 mg/ml petroleum ether extract of leaves and flowers. It is concluded that the leaf extract, when compared to flower extract, exhibits more potency on hair growth. (22)

4.      Clinical evaluation to assess the safety and efficacy of coded herbal medicine “Dysmo-off” versus allopathic medicine “Diclofenac sodium” for the treatment of primary dysmenorrhoea: - The clinical study on primary dysmenorrhoea to comparatively examine the coded herbal drug formulation “Dysmo-off” with authentic allopathic medicine “Diclofenac sodium”. A random controlled clinical trial was conducted. These evaluations were based on verbal rating scale so as to ascertain the rate of analgesic effects on dysmenorrhoeic pain. The patients were randomly allocated with the ratio of 1:2 for controlled treatment with (NSAIDS) (n=40) received Diclofenac sodium tablets twice daily for 4 days (50 mg one day prior to and Three Days after the menstruation), and test treatment with Dysmo-off (n=80) received powdered Dysmo-off twice daily for 4 days (5 g one day prior to and three days after the menstruation). Treatment lasted for 4 consecutive menstrual cycles. Haemoglobin, ESR and ultrasound were measured at baseline during study. All subjects were clinically studied.(23)

5.      Thermographic evaluation: - In the present study, the authors used thermography to evaluate the effects of herbal formulations based on “Sho” scientifically. In the cases that were suitable for Keishibukuryogan, the so called Keishibukuryogan Sho, a significant skin temperature rise was observed in the upper half of the body after the intake of Keishibukuryogan. In a case that was suitable for Hochuekkito, a marked elevation of skin temperature spread through the upper trunk. It suggested that thermography is useful for an objective evaluation of Sho in Kampo medicines, and for identification of the action site of the herbal formulation.(24)

6.      Biochemical evaluation: - Most of the herbal drugs are a mixture of a number of ingredients. Their cumulative effect increases the efficacy of the drug in curing the diseases. Muthu Marunthu is an herbal formulation comprising of eight various plant ingredients, and has been claimed to possess anticancer effect. It was observed that the growth rate in rats was normal and there was no change in blood parameters such as glucose, urea, proteins, cholesterol and also in the activities of pathophysiological enzymes such as lactate dehydrogenase (LDH), gluconate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), alkaline and acid phosphatase after Muthu Marunthu administration. The tumor weight was found to be reduced in methylcholanthrene induced fibrosarcoma rats after Muthu Marunthu treatment. (25)

7.      Evaluation of Kutaj-Ghanavati for alkaloidal principles:- Kutaj-Ghanavati is a reputed Ayurvedic preparation used in dysentery and diarrhea. It contains water extract of Kurchi bark and fine powder of aconite roots. It was evaluated quantitatively and qualitatively employing TLC and titrimetric method. In TLC study no interference of Kurchi and Aconite alkaloids with one another in their respective solvent systems. The formulation was found to contain all alkaloids of Kurchi and Aconite. (26)

8.      Organoleptic evaluation: - Organoleptic evaluation of food products plays an important role in judging the censoring acceptability or rejection of food items in the market. Effect of various treatments (blanching, pricking, and lye treatment), sugar concentration (50%, 60%, 70%) and storage on the color scores; flavor scores; texture scores of intermediate moisture apricots. The overall acceptability of the products was significantly higher in 70% sugar syrup but these scores decreased as the storage period advanced. (27)

 CONCLUSION: - The subject of herbal drug standardization is massively wide and deep. There is so much to know and so much seemingly contradictory theories on the subject of herbal medicines and its relationship with human physiology and mental function.

   For the purpose of research work on standardization of herbal formulations, a profound knowledge of the important herbs found in India and widely used in Ayurvedic formulation is of utmost importance.(6)

    Even when the chemical composition of a plant extract is known, the pharmacologically active moiety may not be. Environment, climate, and growth conditions influence composition, as does the specific part of the plant and its maturity. Monographs detailing standardization of active ingredients would improve the marketplace. Even if an herbal product is standardized to, for example, 4% of a constituent, the remaining 96% of ingredients is not standardized and may affect the product’s solubility, bioavailability, stability, efficacy and toxicity. Just as controlled trials are necessary to establish safety and efficacy, manufacturing standards are required to ensure product quality.(28)

  Now a days newer and advanced methods are available for the standardization of herbal drugs like fluorescence quenching, combination of chromatographic and spectrophotometric methods, biological assays, use of biomarkers in fingerprinting etc. Bioassay can play an important role in the standardization of herbal drugs and can also become an important quality control method as well as for proper stability testing of the product.(4)

  India can emerge as the major country and play the lead role in the production of standardized, therapeutically effective ayurvedic formulation. India needs to explore the medicinally important plants. This can be achieved only if the herbal products are evaluated and analyzed using sophisticated modern techniques of standardization such as UV- visible, TLC, HPLC, HPTLC, GC-MS, spectrofluorimetric and other methods.(6)

 

    References

1. Sagar Bhanu P.S., Zafar R., Panwar R., “Herbal drug standardization”, The Indian Pharmacist, vol. 4(35), May 2005, 2005, pp.19-22.
2. Patel P.M., Patel N.M., Goyal R.K., “Evaluation of marketed polyherbal antidiabetic formulations uses biomarker charantin”, The Pharma Review, vol.4 (22), June 2006, pp.113.
3. Patel P.M., Patel N.M., Goyal R.K., “Quality control of herbal products”, The Indian Pharmacist, vol.5(45), March 2006, pp.26-30.
4. Bhutani K.K., “Herbal medicines an enigma and challenge to science and directions for new initiatives”, Indian Journal of Natural Products, vol.19 (1), March 2003, pp.3-8.
5. Kokate C.K., Purohit A.P., Gokhale S.B., “Analytical pharmacognosy”, Pharmacognosy, 30th edition, Feb. 2005, pp.1,99.
6. Shrikumar S., Maheshwari U., Sughanti A., Ravi T.K., “WHO guidelines for herbal drug standardization”, 2006.
7. Ansari S.H., “Standardization of crude drugs”, Essentials of Pharmacognosy, Ist edition, 2005-06, pp.14, 581.
8. Gokhale S.B., Surana S.J., “Fluorescence quenching as a tool for identification and quality control of crude drugs”, Planta indica, vol 2 (3), July 2006, pp.47.
9. Shaw P.C., Pui-Hat Butt P., “Authentication of Panax species and their adulterants by random primed polymerase chain reaction”, Planta Medica, vol. 61, 1995, pp.466-469.
10. Lazarowych N.J., Pekos P., “Use of fingerprinting and marker compounds for identification and standardization of botanical drugs: Strategies for applying pharmaceutical HPLC analysis to herbal products”, Drug Information Journal, Vol.32, 1998, pp.497-512.
11. Apers S., Naessens T., Pieters L., Vlietinck A., “Densitometric thin-layer chromatographic determination of aescin in an herbal medicinal product containing Aesculus and Vitis dry extract”, Jr. of Chromatographic Analysis, vol.1112(1-2), April 2006, pp.165-170.
12. Nair V.D., Kanfer I., Hoogmartens J., “Determination of stigmasterol, beta-sitosterol and stigmastanol in oral dosage forms using HPLC with evaporative light scattering detection”, Journal of pharmaceutical and biomedical analysis, vol. 41(3), June 2006, pp. 731-737.
13. Sarmani S.B., Abugassa I., Hamzah A., Yahya M.D., “Elemental analysis of herbal preparations for traditional medicines by neutron activation analysis with the kO standardization method”, Biological trace element research, 1999, pp. 365-376.
14. Careri M., Elviri L., Mangia A., “Liquid chromatography-UV determination and liquid chromatography-atmospheric pressure chemical ionization mass spectrometric characterization of sitosterol and stigmasterol in soyabean oil”, Jr. of Chromatographic Analysis, vol. 935(1-2), Nov.2001, pp.249-257.
15. Yu B.S., Lai S.G., Tan QL, “Simultaneous determination of cinnamaldehyde, eugenol and paeonol in traditional Chinese medicinal preparations by capillary GC-FID”, Chemical and pharmaceutical bulletin, vol. 54(1), Jan 2006, pp. 114-116.
16. Santosh M.K., Shaila D., Sanjeeva Rao I., “Standardization study of dadimastaka and pushyannga churnas”, Asian Jr. of Chemistry, vol. 16(34), 2004, pp. 1735-1741.
17. Azamathulla Shaik, Hule Amolkumar, “Evaluation of adaptogenic activity profile of herbal preparation”, Indian Jr. of Experimental Biology, vol. 44, July 2006, pp.574-579.
18. Zaporozhets O.A., Lipkovska N.A., “A new test method for the evaluation of total antioxidant activity of herbal products”, Jr. of Agricultural and Food Chemistry, vol. 52(1), 2004, pp.21-25.
19. De Souza T.P., Zulian Lionzo M.I., “Evaluation of microbial contamination reduction on plants through technological process of decoction and spray dry”, Brazilian Jr. of Pharmacognosy, vol. 16(1), 2006, pp.94-98.
20. Basu S., Hazra B., “Evaluation of nitric oxide scavenging activity of selected medicinal plants used in inflammatory diseases”, Phytotherapy research, vol. 20(10), 2006, pp. 896-900.
21. Shinde A.D., Bhise S.B., “Evaluation of wound healing activity of herbal drug combination of Tridax Procumbens, Azadirachta indica, Curcuma longa and Apis mellifera”, Indian Drugs, vol. 41(6), June 2004, pp.376-378.
22. Adhiraj N., Ravikumar T., Shanmugasundaram N., “In vivo and in vitro evaluation of hair growth potential of Shoe flower”, Jr. of ethanopharmacology, vol. 88(2-3), 2003, pp. 235-239.
23. Nazar H., Usmanghani K., “Clinical evaluation toi assess the safety and efficacy of coded herbal medicine “Dysmo-off” versus allopathic medicine “Diclofenac sodium” for the treatment of primary dysmenorrhoea”, Jr. Herb Pharmacotherapy, vol. 6(1), 2006, pp.21-39.
24. Inokawa M., Iguchi K., Kohda H., “Thermographic evaluation of the efficacy of Kampo medicine”, Hiroshima Jr. Med Sci., vol. 55(1), March 2006, pp.1-8.
25. Palani V., Senthilkumaran R.K., “Biochemical evaluation in antitumour effect of Muthu Marunthu on experimental fibrosarcoma in rats”, Jr. of Ethanopharmacology, vol. 65(3), 1999, pp.257-265.
26. Bhavasar G.C., Pundarikakshudu K., “Evaluation of Kutaj-Ghanvati for alkaloidal principles”, Indian Jr. Natural Products, vol. 20(1), 2003, pp.33.
27. Sharma H.R., Verma P., “Organoleptic and chemical evaluation of osmotically processed Apricot wholes and halves”, Natural Product Radiance, vol.5 (3), Sep-Oct 2006, pp.350-356.
28. Boullata I.J., Nace M.A., “Safety issue with herbal medicine”, Pharmacotherapy, vol. 20(3), 2000, pp.257-269.

About the Author

M.Pharm.(Pharmacognosy)
Lecturer at BN Girls college of Pharmacy, Udaipur-INDIA

Where Should I Get My Mickey Mantle Autographed Baseball Authenticated?

I have a baseball that was autographed in person by Mickey Mantle. It is 100% authentic. I am looking to sell it because I was recently laid off from work and money is very tight. I want to sell it on ebay but, in order to make a fair amount of money it has to be authenticated/have a certificate of authenticity.

I already checked with a company called PSA/DNA and it's going to cost me $100 just to get it authenticated. If I could afford the $100 to have this done I wouldn't be selling it in the first place.

So is there anyone out there who knows what I can do? Is there another place to have this done? Can I get it done for cheaper?

THANKS for responding!!

If that $100 raised the perceived value of the ball from $300 to $1500, is it worth it?

Even grading services have perceived value. If there is a top grader where people will know everything is legit 100% of the time vs. one that is only right 80% of the time. It makes a difference.

Paul Newman and Joanne Woodward in Westport, CT

Authentic Autographed

How To Tell If the Signatures on Old Stock Certificates are Authentic

How can you determine if the signatures on a collectible stock certificate are authentic autographs?  First, there's good news.  Scripophily (the collecting of antique stock certificates and bonds) does not generally have the rampant forgery or mechanized signature issues of some other collectible fields (e.g., signed sports collectibles).
 
The question to be answered is whether the signature was Hand Signed rather than printed or stamped.  An expensive paper and ink chemical analysis is seldom needed.  The following can help in determining originality:

1. The older the document is, the more likely it was hand signed.  In fact, in the 1800s, a personal and artful signature was the norm.

2. Certificates normally have multiple signatures (President, Secretary, Transfer Agent, owner, witness, etc.).  The signatures and any notations should be in different handwriting for each person and, often, different colorations and applications of ink or pencil.

3. Research the document using books and dealers' or hobbyist's websites (such as coxrail.com for railroads).  You may find authentication comments or images you can compare with your document.

4. If you have more than one certificate or image for comparison, look for small variations - that's good.  If the signatures would match exactly if overlaid, they're probably reproduced.

5. Since older certificates are often on thinner paper, an original signature may appear darker than the printed portions of the certificate when it is viewed from the back.

6. Sometimes a pen signature will have left a very slight depression in the paper which can be seen from the front or back when held at an angle to a light.

7. If the placement of the signature is contrary to the layout of the print, e.g., it covers part of the design instead of being perfectly placed, that may indicate originality (though not always).

8. Early signature ink sometimes "bled" slightly out into the paper from the written lines.  You may be able to see this with a magnifier.

A couple of important final points to remember:

Firstly, it is possible to prove an autograph is not authentic, but it is impossible it prove it is authentic.  Every statement of authenticity about a signature (be it Marilyn Monroe, Michael Jordan or George Washington) is a statement of informed (we hope) opinion.

The only way to be 100% sure is if you personally watched the person sign your item and then kept the item in your possession.  That seldom is an option and never is for antique items.

Secondly, because of the first point, you should always purchase collectible items from reputable dealers who offer a reasonable return policy.  You should be happy with what you acquire.

Whether you even care about the signature is completely up to you, of course, but it can be one of the research paths that lead to a sense of discovery as you explore the historical background and personal stories behind your latest stock certificate acquisition.

About the Author

Visit www.RealStockCertificates.com for better quality antique old stocks at better prices. Professional Authors at:
www.Article-Writing-Services.com

If you could have one authentic autographed ball, who would it be?

(and you can't sell it)

Jackie R for me.

Mickey Mantle or Lou Gehrig.

Az's Ace Authentic Autographed Tennis Balls